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Fluorescence Ìý Leica resourcesÌý High Content Imaging/ Analysis
General Microscopy
Websites:
(FSU),Ìý (Olympus),Ìý (Zeiss) and (Nikon). Four quite exhaustive sites (very similar in content and organization), covering everything from basic microscopy to more specialized subjects.
(Leica), less organized but searchable collection of articles, webinars and tutorials on a variety of subjects.
, short articles and company-sponsored webinars covering a variety of different microscopy-related subjects.
, lecture videos from basic microscopy to specialised applications.
Some introductory reviews:
(Nature Methods)
(Nature Methods)
(Nature Methods), (Journal of Microscopy)
(Nature Biotechnology)
(Journal of Microscopy)
Image quality:
(Journal of Cell Biology)
(Journal of Cell Biology)
Fluorescence tools
Interactive databases of fluorophore excitation/ emission curves and filters:
(Life technologies), covers mainly their offering of fluorophores, allows to customize the filter specifications.
(Chroma) and (Semrock), both are filter manufacturers and only list their filter-sets. Their databases contain many fluorphores that are not listed in the Spectraviewer. ÌýÌý
(Chroma), a compilation of filter design and terminology.
2-photon excitation:
(drbio.cornell)
(Nature methods)
Fluorescent dyes and techniques:
(ThermoFisherScientific)
(Leica)
(ThermoFisherScientific)
(BOC Sciences)
Fluorescence and IHC reference images:
Primary antibody search:
Leica resources
Information about Leica microscopes
, explains the objective nomenclature and objective specifications.
, searchable Leica microscopy resources site with articles, webinars, tutorials.
, free download, allows to view .lif images and save them in open image formats.
PMT and HyD detectors, explains how Hybrid detectors offer higher sensitivity than standard PMT detectors.Ìý
High Content Imaging/ Analysis
(PerkinElmer), High Content Screening page with examples for cellular assays and analysis methods.
, introduction to High Content Imaging/ Screening and best practices for assay development.
(Enzo)
(Enzo)
(Journal of Biomolecular Screening), commonly used simple statistical method to evaluate HTS/ HCS assays.
Bioanalysis Ìý ÌýMicroarray Ìý qPCRÌý ÌýPyrosequencing Ìý GeneSpring Ìý Agilent Genomics Workbench Ìý Ìý Ìý Ìý
Bioanalysis
Ìý
Bioanalyzer Reagents Selection Guide
Ìý
Microarray
One-color Gene expression microarray workflow
Ìý(Nature)
Ìý
qPCR
Ìý
(Neuro)
Ìý(Life Technologies)
Ìý(Applied Biosystems)
-Minimum Information for Publication of QuantitativeÌýReal-Time PCR Experiments
Ìý(Journal of biomolecular techniques)
Ìý(Exiqon)
Ìý
Pyrosequencing
Ìý
PyroMark Q24 and Q96-MD Reagents and Consumables
Ìý(Nature Protocols)
Ìý(Biotechniques)
Ìý(Biotage)
Ìý(Standford University)
Ìý(Standford University)
Ìý
GeneSpring software
: Video Tutorials, Webinars, Demo Datasets
: Catalog and Custom Microarray designs
Ten pitfalls of microarray analysis-Agilent technical notes
(Agilent)
Ìý
Agilent Genomics workbench software
Ìý(Agilent)
Ìý(Agilent)
Ìý(Agilent)
in situ RNA detection and Immunohistochemistry
Tecan GenePaint in situ Hybridization Workflow
Ìý(Roche)
Tecan GenePaint automated immunohistochemistry workflow
Ìý(Abcam)
Automation and Transfection/ Injection
Ìý
(PerkinElmer)
(Bio Rad)
(CUSABIO)