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Optical Imaging Facility

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REQUEST MICROSCOPE TRAINING

USER INFORMATION & RULES

FEE STRUCTURE

The CFTRc has 4 state-of-the-art light microscopes to help answer several questions about CF research. These microscopes allow researchers to generate high quality images and quantitative data for high level publication, elevating research projects to the next level.

SOME OF THE SERVICES OFFERED:

  • Live cell imaging
  • Confocal imaging
  • Differential Interference Contrast (DIC)
  • Fluorescence Correlation Spectroscopy (FCS) to study ligand binding in solution and in cells
  • Total Internal Reflected Fluorescence (TIRF)
  • Quantitative imaging of protein and vesicle dynamics
  • Measurement of intra and extracellular pH andÌýCa2+
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​OUR EQUIPMENT INCLUDES:

- the last generation of laser scanning confocal microscope (LSM780, Zeiss) mounted on an inverted Axio Observer fully motorized microscope; equipped with 3 high NA/DIC objectives (20x, 40xW, 63xOil)

- a Spinning-Disc/TIRF microscope (Zeiss) mounted on an inverted Axio Observer fully motorized microscope with an incubation unit; equipped with a 63x Oil objective designed for TIRF experiments

- two ratiometric wide-field imaging systems (PTI inverted microscope, Olympus and Axio Examiner upright, Zeiss).
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HOW TO GET STARTED:

  1. Fill out a Training Request Form
  2. Set up a consultation time with one of our imaging experts
  3. Fill out a to gain access to our microscopy rooms
  4. Complete your training
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For more information, visit the Advanced BioImaging Facility (ABIF) Website HERE.

Supported by:

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Ã山ǿ¼é is situated on the traditional territory of the Kanien’kehà:ka, a place which has long served as a site of meeting and exchange amongst nations. We recognize and respect the Kanien’kehà:ka as the traditional custodians of these lands and waters.

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