Teruko Taketo
Professor
PhD
The sex of an individual is determined by the combination of sex chromosomes, XX or XY, in mammals. Once the gonadal sex is established, however, most of sex characteristics are determined by the hormones produced by the gonad regardless of the chromosomal sex. Exception is the germ line; proper set of sex chromosomes maximizes the fertility of each sex. We are particularly interested in the role of sex chromosomes in female germ cell differentiation, in addition to the mechanism of gonadal sex determination. Our specific aims are; (1) to clarity the mechanism of sex determination and sex reversal in the B6.YTIR mouse strain; (2) to delineate the mechanism of oocyte elimination during the meiotic prophase progression, and (3) to identify the ooplasmic defects in XO and XY oocytes which impair meiotic chromosome segregation. These studies help us understand female infertility, particularly, associated with maternal age.
McClellan, K.A., Gosden, R. and Taketo, T. (2003) Continuous loss of oocytes throughout meiotic prophase in the normal mouse ovary. Developmental Biology 258: 334-348.
DOI: 10.1016/S0012-1606(03)00132-5
Taketo, T., Lee, C.-H., Zhang, J., Li, Y.-M., Lee, C.-Y.G. and Lau, Y.-F.C. (2005) Expression of SRY proteins in both normal and sex-reversed XY fetal mouse gonads. Developmental Dynamics 233: 612-622.
DOI: 10.1002/dvdy.20352
Villemure, M., Chen, H.-Y., Kurokawa, M., Fissore, R.M. and Taketo, T. (2007) The presence of X-and Y-chromosomes in oocytes leads to impairment in the progression of the second meiotic division. Developmental Biology 301: 1-13.
DOI: 10.1016/j.ydbio.2006.10.034
Alton, M. and Taketo, T. (2007) Switch from BAX-dependent to BAX-independent germ cell loss during the development of fetal mouse ovaries. Journal of Cell Science 120: 417-424.
DOI: 10.1242/jcs.03332
Alton, M., Lau, M. P., Villemure, M. and Taketo, T. (2008) The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.YTIR sex-reversed mouse ovary. Reproduction 135: 241-252.
DOI: 10.1530/REP-07-0383
Obata, Y., Villemure, M., Kono, T. and Taketo, T. (2008) Transmission of Y-chromosomes from XY female mice was made possible by replacement of cytoplasm during oocyte maturation. Proceedings of the National Academy of Sciences of the United States of America 105: 13918-13923.
DOI: 10.1073/pnas.0802680105
Park, S., Zeidan, K.T., Shin, J.S. and Taketo, T. (2011) SRY upregulation of SOX9 is inefficient and delayed, allowing ovarian differentiation, in the B6.YTIR gonad. Differentiation 82: 18-27.
DOI: 10.1016/j.diff.2011.04.007
Xu, B.-Z., Obata, Y., Cao, F. and Taketo, T. (2012) The presence of the Y-chromosome, not the absence of the second X-chromosome, alters the mRNAs stored in the fully grown XY mouse oocyte. PLos One 7: e40481.
DOI: 10.1371/journal.pone.0040481
Ene, A.C., *Park, S., Edelmann, W. and Taketo, T. (2013) Caspase 9 is constitutively activated in mouse oocytes and plays a key role in oocyte elimination during meiotic prophase progression. Developmental Biology 377: 213-223.
DOI: 10.1016/j.ydbio.2013.01.027
Moawad, A., Tan, S.L., Xu, B., Chen, H.-Y., and Taketo, T. (2013) L-carnitine supplementation during vitrification of mouse oocytes at the germinal vesicle stage improves preimplantation development following maturation and fertilization in vitro. Biology of Reproduction 88:104, 1-8.
DOI: 10.1095/biolreprod.112.107433
Taketo, T. and Naumova, A.K. (2013) Oocyte heterogeneity with respect to the meiotic silencing of unsynapsed X chromosomes in the XY female mouse. Chromosoma 122: 337-349.
DOI: 10.1007/s00412-013-0415-z
Xu, B., Noohi, S., *Shin, J.S., Tan, S.L. and Taketo, T. (2014) Bi-directional communication with cumulus cells involved in the deficiency of XY oocytes in the components essential for proper second meiotic spindle assembly. Developmental Biology 385: 242-252.
DOI: 10.1016/j.ydbio.2013.11.004
Vernet, N., Szot, M., Mahadevaiah, S.K., Ellis, P.J.I., Decarpentrie, F., Ojarikre, O.A., Rattigan, A., Taketo, T. and Burgoyne, P.S. (2014) The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility. Development 141: 855-866.
DOI: 10.1242/dev.091165
Moawad, A.R., Xu, B., Tan, S.L. and Taketo, T. (2014) L-carnitine supplementation during vitrification of mouse germinal vesicle stage -oocytes and their subsequent in vitro maturation improves meiotic spindle configuration and mitochondrial distribution in metaphase II oocytes. Human Reproduction 29: 2256-2268.
DOI: 10.1093/humrep/deu201
Zhu, J.-Q., Tan, S.L., and Taketo, T. (2017) A lack of coordination between sister chromatid segregation and cytokinesis in the oocytes of B6.YTIR (XY) sex-reversed female mice. Scientific Reports 7:960.
DOI: 10.1038/s41598-017-00922-1
Moawad, A.R., Tan, S.L., and Taketo, T. (2017) Beneficial effects of glutathione during vitrification of mouse oocytes at the germinal vesicle stage on their preimplantation development following maturation and fertilization in vitro. Cryobiology 76: 98-103.
DOI: 10.1016/j.cryobiol.2017.04.002
Nguyen, N.M.P., Ge, Z-J, Reddy, R., Fahiminiya, S., Sauthier, P., Bagga, R., Sahin, F.I., Mahadevan, S., Osmond, M., Breguet, M., Rahimi, K., Lapensee, L., Hovanes, K., Srinivasan, R., Van den Veyver, I.B., Sahoo, T., Ao, A., Majewski, J., Taketo, T., and Slim, R. (2018) Causative genes and mechanism of androgenetic hydatidiform moles. American Journal of Human Genetics 103: 740-751.
DOI: 10.1016/j.ajhg.2018.10.007
Liu, X., Castle, V., and Taketo. T. (2019) Interplay between Caspase 9 and X-linked cellular Inhibitor of Apoptosis Protein (XIAP) in the oocyte elimination during fetal mouse development. Cell Death & Disease 10: 790.
DOI: 10.1038/s41419-019-2019-x
Vaz, B., El Mansouri, F., Liu, X., and Taketo, T. (2020) Premature ovarian insufficiency in the XO female mouse on the C57BL/6J genetic background. Molecular Human Reproduction 26: 678-688.
DOI: 10.1093/molehr/gaaa049
Kazemi, P. and Taketo, T. (2021) Two telomeric ends of acrocentric chromosome play distinct roles inhomologous chromosome synapsis in thefetal mouse oocyte. Chromosoma 130: 41 -52.
DOI: 10.1007/s00412-021-00752-1
Yamazaki, W., Badescu, D., Tan, S.L., Ragoussis, J., and Taketo, T. (2021) Effects of the sex chromosome complement, XX, XO or XY, on the transcriptome and development of mouse oocytes during follicular growth. Frontiers in Genetics 12: 792604.
DOI: 10.3389/fgene.2021.792604.
Yamazaki, W., Tan, S.L., and Taketo, T. (2022) Role of the X and Y chromosomes in the female germ cell line development in the mouse (Mus musculus). Sexual Development 16: 355-364.
DOI: 10.1159/000521151
Kazemi, P. and Taketo, T. (2022) Mouse oocytes carrying metacentric Robertsonian chromosomes have less efficient homologous recombination and higher aneuploidy rate than wild-type oocytes carrying all acrocentric chromosomes. Scientific Reports 12:12028.
DOI: 10.1038/s41598-022-16175-6
Cao, J., El Mansouri, F., Reynoso, S., Liu, Z., Zhu, J., and Taketo, T. (2024) Inefficient Sox9 upregulation and absence of Rspo1 repression lead to sex reversal in the B6.XYTIR mouse gonad. Biology of Reproduction (in press).
DOI: 10.1093/biolre/ioae018 [BIOLRE-2023-0440]
