New laser method reproduces masterworks to protein patterns <br />Canadian scientists reproduce Girl with a Pearl Earring with protein patterning technique
Canadian researchers have created a new protein patterning technique that鈥檚 enabled them to reproduce complex cellular environments and a miniature version of a masterpiece painting.
According to a new study published in the journal Lab on a Chip, scientists from Universit茅 de Montr茅al, the Maisonneuve-Rosemont Hospital Research Centre, and the and the joint 缅北强奸-Montreal Neurological Institute and Hospital NeuroEngineering Program, have developed a laser technology that can mimic the protein patterns that surround cells in vivo and that could lead to great advances in neuroscience.
To illustrate the precision of their protein patterning technique, the research team reproduced a masterwork of Dutch painter Johannes Vermeer, specifically Girl with a Pearl Earring, in the miniature dimension of 200 microns wide or about the thickness of two hairs. The researchers also used their novel technology to replicate the brain鈥檚 complex cellular environment. It鈥檚 a major discovery, since the new laser technology can encourage and guide the growth of finicky nerve cells.
鈥淲e have created a system that can fabricate complex methods to grow cells,鈥� says Santiago Costantino, the study鈥檚 lead author and a scientist at the Universit茅 de Montr茅al and Maisonneuve-Rosemont Hospital Research Centre.
鈥漌e see this technique as being very relevant to neuroscience and immunology research. With this system, we laid down a chemical gradient to guide the growth of nerve fiber, which is very useful in studying nerve damage and repair.鈥�
Flexible and precise:
Using laser-assisted protein adsorption by photobleaching (LAPAP), the scientific team bound fluorescently-tagged molecules to a glass slides and created patterns of proteins similar to those of the human body. They then demonstrated how flexible and precise this technique could be by reproducing a fluorescent micro version of Girl With a Pearl Earring.
鈥淭he flexibility, precision and ease of this technique will hopefully lead to increased access in protein patterning, which could lead to major advances in science,鈥� says Dr. Costantino, who is also a member of the BioFemtoVision Canadian Research Group, which includes researchers from the Universit茅 de Montr茅al and the Institut National de la Recherche Scientifique who are working on developing new laser technologies for vision science.
鈥淥ur next goal is to extend laser-assisted protein adsorption by photobleaching to fabricate more complex protein combinations and distributions,鈥� adds Dr. Costantino. 听鈥淲e want to improve our imitation of the chemical environment found in the early stages of developing organisms.鈥�
About the study:
The article, 鈥淧atterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP," published in , was authored by Santiago Costantino and Jonathan M. B茅lisle of the Universit茅 de Montr茅al and Maisonneuve-Rosemont Hospital Research Centre, as well as James P. Correia, Paul W. Wiseman and Timothy E. Kennedy of 缅北强奸 and the Montreal Neurological Institute.
Partners in research:
This study was funded through grants from the Natural Science and Engineering Council of Canada, the Fonds qu茅b茅cois de la recherche sur la nature et les technologies, Canadian Institutes of Health Research and the Fonds de la recherche en sant茅 du Qu茅bec.
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