Fluorescence lifetime images were acquired on a Zeiss LSM710 microscope outfitted with a PicoQuant LSM FLIM upgrade kit (...). The excitation source was a 473 nm pulsed laser (PicoQuant LDH series) operated at a 50 MHz pulse rate (time resolution, 400 ps)
FLIM images and histogram of B16 cells incubated with free Dox ()
